Items | OD450 value | Control Result for Valid Assay |
Positive Control | < 0.3 | Passed |
Negative Control | > 0.9 | Passed |
Items | Cutoff | Result | Interpretation |
Neutralizing antibody test | ≥20% | Positive | Neutralizing antibody detected |
<20% | Negative | No detectable neutralizing antibody |
Problem | Probable Cause | Solution |
Poor Precision | Wells are not washed or aspirated properly | Make sure the wash apparatus works properly and wells are dry after aspiration |
Bubbles in the wells | Tap plate gently to disperse bubbles | |
Wells are scratched with pipette tip or washing needles | Dispense and aspirate solution into and out of wells with caution | |
Particulates are found in the samples | Remove any particulates by centrifugation prior to the assay | |
High background | Plate is not washed properly | Make sure the wash apparatus works properly |
Incorrect incubation times and/or temperatures | The OD value increased gradually along with the time.Reduce the color developing time properly | |
Weak/No Signal | Pipetting errors | Make sure the pipette is calibrated |
The working solution not be prepared immediately before use | The working solution should be prepared immediately before use and should not be stored | |
Volumes errors | Repeat assay with the required volumes in manual | |
The plate is not incubated for proper time or temperature | Follow the manual to repeat assay | |
Detection A working solution is not completely mixed with the samples | After adding the Detection A into the wells, make sure the detection A and the samples are mixed thoroughly |
MATERIALS PROVIDED & STORAGE CONDITIONS
PART | Format | Description | STORAGE |
Capture Plate | 1 plate | 96 well polystyrene microplate (12 strips of 8 wells) coated with RBD( E484Q,L452R)protein. | Store in sealed at - 20℃. |
Positive Control | 1 vial | 12μL/vial, 1mg/mL antibody specific for RBD protein with preservatives, 1:100 diluted by dilution buffer before used. Final concentration was 10μg /mL. | Store at - 20℃. |
Negative Control | 1 vial | 60μL/vial, negative control with preservatives, 1:10 diluted by dilution buffer before used. | Store at - 20℃. |
Detection A | 1 vial | 12μL/vial HRP labeled ACE2 protein (including preservative) , 1:2000 diluted by dilution buffer before used. | Store at - 20℃. |
Sample Dilution Buffer | 1 bottle | 25 ml/bottle diluent (including preservative) was used to dilute the Positive Control ,Negative Control ,Serum and Plasma. | Store at 4℃. |
Reagent Dilution Buffer | 1 bottle | 25 ml/bottle diluent (including preservative) was used to dilute the Detection A. | Store at 4℃. |
Wash Buffer Concentrate | 1 bottle | 25 mL/bottle of a 20-fold concentrated solution of buffered surfactant with preservative. 1:20 diluted by deionized water before used. | Store at 4℃. |
Color Reagent TMB | 1 bottle | 12 mL/ bottle of TMB(tetramethylbenzidine) . | Store at 4℃. |
Stop Solution | 1 bottle | 6 mL/ bottle. | Store at 4℃. |
Plate Sealer | 4 strips | Adhesive strips. | RT. |
* Provided this is within the expiration date of the kit.
Guarantee and Disclaimer:
After receiving the product, if finds that the product is mismatched, damaged or missing components, please keep the original package and submit the objection to the company by mail within seven working days. Failure to file an objection within the time limit is considered qualified.
When the buyer keeps the product, it should be kept in accordance with the storage conditions shown in the product label and the manual. If the product quality is caused by improper storage, it will not be guaranteed.
When the buyer tests the product, they should be submitted by the beginning of use when found the quality issue, rather than when the product was used or used up, in order to prepare our products for recycling and confirm the quality of the products. If it is the quality problem, our company is responsible for exchange or return. In the event of a claim, our company will compensate the discretion within the scope of the product price limit and will not accept any part of the value of the product itself. Since the date of receipt, the product has not been reflected for more than three months and should not be returned.
The products provided by our company are for research use only and should not be used for clinical diagnosis or treatment. If a unit or individual changes the use of our products without authorization, we will not bear any responsibility.