Technical Service

Molecular interaction analysis

Service Brief

BiacoreTM, MicroCalTM ITC and MicroCalTM DSC three molecular interaction analysis systems are available To provide unique molecular interaction analysis services according to different needs. efficient, high sensitivity, affordable, AtaGenix is your best choice.

  Affinity is an important parameter for determining the interaction between molecules, and is an important indicator for understanding molecular recognition and biological processes, drug discovery and screening. For the evaluation of drug efficacy, the stability of biomacromolecules and their complexes, etc., it is necessary to conduct a comprehensive study on kinetics, thermodynamics and thermal stability, including the intermolecular binding, the speed and strength of binding, the mechanism of the combination and so on.

BiacoreTM

 

BiacoreTM is based on the new biosensing analysis technique developed by physical optical phenomenon called surface plasmon resonance. AtaGenix has two systems, BiacoreTM X100 (single channel) and BiacoreTM T200 (4-channel, 384 continuous injection), which provide superior kinetic testing services.


Biacore单通道生物传感分析系统Biacore4通道生物传感分析系统

 

BiacoreTM X100                       BiacoreTM T200



Type of information

Affinity, binding kinetics, quantification, specific binding activity, stability and specificity

 

AtaGenix Advantages

1. High-end platform for interaction analysis between basic research and new drug research

2. Real-time interaction of biomolecules without labeling

3. No molecular weight limitation for small molecules involved in interaction

4. Wider range of kinetic parameters allows for faster binding and slower dissociation

5. Continuous injection analysis of 384 samples

6. Less sample and target dosage (ug level)

7. Automation, high sensitivity, high throughput

MicroCalTM ITC200

  Isothermal titration calorimetry (ITC) is the thermodynamic technique that monitors any chemical reaction initiated by the addition of the binding component. For researchers who need to use existing methods for solid phase hardening or use thermodynamic parameters for molecular design of new drugs, BioCalTM iTC200 isothermal titration microcalorimeter can study the interaction between biomacromolecules from thermodynamic, providing the thermodynamic mechanism for the interaction of biological macromolecules.

MicroCal等温滴定微量热仪 

MicroCalTM iTC200

Type of information

Binding constant, amount of binding sites, molar binding enthalpy, molar binding entropy, molar constant pressure heat capacity and kinetic parameters (eg enzyme activity, enzymatic reaction Michaelis constant and number of enzyme conversions)


AtaGenix Advantages

1. Fast get KD

2. Measuring target activity

3. Confirm the combination of drug and target

4. Using thermodynamics to guide the optimization of lead compounds

5. Confirm IC50 and EC50 values

6. Determination of enzyme kinetics

MicroCalTM DSC

   Differential thermal scanners (DSCs) measure the thermodynamic changes in molecular interactions by increasing or decreasing the temperature to induce changes in the polymer, the preferred technique for characterizing the thermal stability of molecules. MicroCal VP-Capillary DSC provides with the fastest and most accurate identification of the most stable protein and biopharmaceutical candidates.

MicroCal VP-Capillary DSC系统 

MicroCal VP-Capillary DSC

 



Type of information

Thermal stability

 

AtaGenix Advantages:

1. Fast determine the best stability conditions

2. Label-free study of molecules in their natural state to achieve direct measurement of biomolecular stability in solution

3. High throughput, high sensitivity

Consultation and ordering

Tel: 0086-27-8743 3958

Cell-phone number+8618571544060

Email:info@atagenix.com