There are two main ways to produce large-scale monoclonal antibodies, one is the ascites preparation method, and the other is the transfer bottle culture method.
Ascites Preparation Method: Hybridoma cell lines are cultured in vitro to a certain concentration, and then the hybridoma cells are injected into the abdominal cavity of mice to produce ascites, from which monoclonal antibodies are purified. Each mouse can produce 3~8mL of ascites, and the antibody content can reach 1~10mg/mL. the period of ascites preparation is 3~4 weeks. Ascites preparation method features with low cost, high yield and fast time, which is the main large-scale antibody production method at present.
Transflask Culture Method: Hybridoma cells are cultured in large scale in vitro, and the antibody is purified from the culture medium. Antibody content reaches 20~80mg/L. Transflask preparation method has the advantages of high antibody quality, no mouse heteroprotein, and no animal virus, etc.
AtaGenix provides high quality monoclonal antibody production and purification services.
1. Professional SPF grade animal laboratory and large-scale sterile flask cell culture incubator.
2. Rapid and efficient production of ascites and hybridoma culture supernatant.
3. Professional antibody purification team to provide follow-up purification service for ascites and hybridoma culture supernatant.
Balb/c Mouse Ascites Production Purification
Hybridoma cell lines with at least 80% cell viability
Hybridoma cell initial medium brand, catalog number, and preparation method
Purified monoclonal antibody, purity at least 90% final purification experiment report
Cell trans-flask culture purification
Cell Trans-Flask Culture Purification