Technical Service

Antibody humanization

Service Brief

Antibody humanization refers to the humanization of most amino acids of murine monoclonal antibodies by DNA recombination and protein engineering to reduce heterogeneity and retain the affinity and specificity of the parental mouse monoclonal antibodies. The antibody expressed by recombinant gene has both murine component and human component, so it is called humanized antibody.

抗体人源化设计

Advantages

1. Reduce the immune rejection.

2. More efficiently raise effect factors or effector cells

3. Long half-life, improves the pharmacokinetics of the antibody.


Basic rules

1. Maintain or increase the affinity and specificity of the antibody.

2. Reduce or substantially eliminate the immunogenicity of antibodies and reduce immune rejection

category

1. Chimeric antibody:

  The light and heavy chain variable region genes of heterologous antibody are inserted into an expression vector containing human antibody constant region by DNA recombination technology, and the mammalian cells are transfected to express the chimeric antibody.


2. Modified antibody

  The modified antibody is also called CDR grafting antibody, and CDR of the variable region of the antibody is the region in which the antibody recognizes and binds to the antigen, and directly determines the specificity of the antibody. Transplantation of CDRs of the murine monoclonal antibody into the human antibody variable region, in place of human antibody CDR, allows the human antibody to obtain the antigen binding specificity of the mouse monoclonal antibody while reducing its heterogeneity.


3. Fully humanized antibody

  Fully humanized antibody refers to the human antibody gene that is transferred to a genetically engineered antibody gene-deficient animal by transgenic or transchromosomal technology, so that the animal expresses the human antibody and achieves full humanization of the antibody.

AtaGenix advantage

1. With an experienced technical team which has developed the therapeutic antibody drugs in the market.

2. Independent-developed high expression vectors and cell lines, as well as cell lines with commercial licenses.

3. From cell line development, to optimization of fermentation culture conditions, to 150L cell tank fermentation, the complete development cycle.

 

Program and leading time

Stage

 

Service

Time(week)

1

 

Antibody Sequencing

4

2

Design of Humanized antibody

2~4

3

 

Gene Synthesis, Cloning

6~8

4

 

Transient Transfection and Purification of Humanized Antibody

6~8

5

 

Analysis(SPR and Epitope mapping)

12~14

6

 

Stable Cell Line Developmet

24

 

service content


Cat. No.

Item

 Leading time

(¥)Price ($)

ata602

Antibody humanization

Enquire

Enquire



Tel: 0086-27-8743 3958

Cell-phone number+8618571544060

Email:info@atagenix.com