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Current Position：Technical Service / Protein / B.Subtilis Expression System / B.Subtilis Expression System
Technical Service
Protein
Antibody
Therapeutic Antibody Development
Recombinant Antibody Expression

B.Subtilis Expression System

Introduction

1. Simple cell wall, and the secreted protein products will not be mixed with endotoxin.

2. Able to secrete a large amount of extracellular target proteins, easy to recover and purify.

3. A set of highly efficient secretion signal peptide and molecular chaperone system can complete the efficient secretion and expression of target proteins and avoid the formation of inclusion bodies.

Advantages

1. We have a variety of expression vectors for B. subtilis, such as pHT01, pHT43 and pHT254.

2. There are many different expression hosts, such as AS1, 1012 and BS168 for intracellular expression, and WB800N for secretion; Meet different requirements of intracellular or secretion expression.

3. Highly efficient bacillus subtilis sensory state can be prepared.


Project

Receivables

Contents

Time Frame

Deliverables

 

 

B.Subtilis Expression System

 

 

Gene sequence

Vector 

Strains

Gene synthesis

2-3 weeks

Gene synthesis reports

Expression  purification test

2 weeks

Expression & purification test reports

1L expression & purification

2 weeks

Protein samples & reports

Fermentation & purification

1-4 weeks

Purified protein & reports

Case Study

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