Rabbit monoclonal antibody features with high affinity, high specificity, high sensitivity, recognizing more novel epitopes, and easy to humanize, etc. Rabbit monoclonal antibody development and treatment for antibody drugs has a very broad prospect, and the launch of rabbit monoclonal antibody drugs opens a new chapter in the research and development of antibody drugs with an unlimited development potential.
The rabbit monoclonal antibody preparation service adopts phage display technology, directly selecting a variety of targets from the rabbit-derived natural antibody library with high library capacity. Rabbit immune antibody libraries for one or several target proteins can be customized according to customer needs, screen high-affinity rabbit monoclonal antibody sequences, and ultimately recombinantly express them into complete rabbit monoclonal antibodies.
Diversified 10 billion level rabbit-derived scFv natural antibody libraries:
1. Customized Antibody Libraries: The improved two-step library construction program can minimize the number of erroneous sequences and ensure the diversity distribution of library.
2. High-quality & Multi-dimensional Library QC Report: Fully reflect the diversity of library distribution
3. Accept multiple types of target antibody screening: proteins, peptides, small molecules, cell surface proteins , etc
4. Customized panning solutions according to project requirements: solid-phase panning, liquid-phase panning and cell-based panning are available, and can be combined according to specific project requirements to maximize the success rate of panning.
Projects | Receivables | Contents | Time Frame | Deliverables | |
Rapid Rabbit Monoclonal Antibody Preparation | Phage Screening Route | Antigen Sequence | Naïve Library Screening | 3-4 Weeks | Delivery of 1-3 Sequences |
Immunobanking & Screening QC Analysis | 10-14 Weeks | Phage Immune Libraries (Phage Greater Than 5*10^8 Pfu) 5-10 Positive Strains 5-10 Antibody Sequences Technical Service Reports | |||
Single B Screening Route | Antigen Preparation Animal Immunization Splenocyte Isolation & Positive B-Cell Screening Positive Clone Amplification & Recombinant Expression Recombinant Antibody Mass Expression | 20-24 Weeks | 100µg/Strain Monoclonal Antibody pUC Plasmid & Sequence Antibody Sequences for 3 Strains Technical Service Reports |
Note: Phage natural rabbit scFv libraries can also be selected for direct panning. Accepted antigen types include proteins, peptides, small molecules and cell surface proteins.
Optional Services: Antigen preparation/full-length and antibody fragment production/affinity sequencing/affinity maturation/bispecific antibody development/stable cell line construction
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