Technical Service

Mouse Monoclonal Antibody Pair Development


The basic principle of Sandwich ELISA is to immobilize a certain amount of coated antibody on the surface of polystyrene microtiter plate by physical adsorption, add sealing protein to close the unbound site, then add the sample containing antigen to be tested, and then add enzyme-labeled specific antibody to develop the color with TMB substrate, and the color of the microtiter plate is positively correlated with the concentration of the substance to be tested. This method is suitable for the determination of bivalent or more than bivalent large molecule antigens, not suitable for the determination of semi-antigens and small molecule monovalent antigens, because they can not form a two-site sandwich.The most critical part of the Sandwich ELISA is to capture the antibody and detect the antibody pairs formed by the antibody, and PHUKEN Bio can provide the service of preparing the antibody pairs in the Sandwich ELISA method.


Schematic Diagram of the Sandwich ELISA Experiment


1. Five protein expression promotes antigen preparation

2. Experienced in ELISA developed by AtaGenix

3. Customized experimental solutions

4. ELISA, chemiluminescence platforms and other applications screening




Time Frame


Antigen Information

Antigen  Preparation: Peptide/protein

Immunization: Mouse(Optional Rat)

Fusion & Screening

Subcloning & Screening

Production of monoclonal antibodies (5-10 strains)

Antibody labeled biotin

Pairing assay: recombinant protein

5-6    Months

Purified monoclonal antibody 2mg each (customized quantity)

Hybridoma cell line (at least 1 pair of cell lines)

Antigen sample 200ug

Experiment report

Optional services: hybridoma cell line sequencing/full-length antibody production/affinity assay/stable cell line construction

Case Study