IdeZ Protease

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  • Product overview
  • Product performance
  • Standard Operating Procedure
  • How to order
  • Instructions
Product Nameicon
IdeZ Protease
Catalog No.icon
Expression hosticon
Restriction sitesicon
The protease has very high substrate specificity, can only recognize IgG, and digest at specific site in the lower hinge region of the antibody to hydrolyze IgG into intact F (ab ') 2 and Fc fragments.
38 kDa
>95% as determined by SDS-PAGE quantitative densitometry by Coomassie Blue Staining.
Enzyme activityicon
The unit enzyme activity of different batches of IdeZ protease can be determined by SDS-PAGE of cleavage of recombinant IgG.
Unit Definitionicon
The amount of enzyme required to shear 1μg of recombinant monoclonal IgG> 95% at 37°C for 30 min is defined as an active unit.
Digestion reaction conditionicon
37℃, 30min
N-terminal His Tag
PBS pH7.5, 50% glycerol
Store at -20 °C for 12 months from the date of receipt.


1. Add appropriate amount of IgG (maximum amount 5mg) to digestion buffer or other suitable buffer;

2. Add IdeZ protease to IgG samples: add 1 unit of IdeZ per 1μg IgG;

3. Incubate the sample at 37 ° C for 30-60min. IdeZ proteases are most active in buffers at or near neutral pH. The recommended reaction buffer is 50 mM sodium phosphate, 150 mM NaCl (pH 6.6). Many common biological buffers such as Tris or PBS can also be used. When using a buffer (such as acetate buffer) outside this pH range, the incubation time or enzyme amount needs to be optimized according to the actual situation.


1. Ideal concentration of IgG: 1-20mg/ml;

2. IdeZ protease efficiently cleaves human, humanized, chimeric, sheep, rabbit and monkey IgG and mouse IgG2a and IgG3. IdeZ protease will also cleave many Fc fusion proteins and antibody-drug conjugates (ADC);

3. IdeZ protease does not cleave mouse IgG1 / IgG2b, rat, pig, cow or goat IgG. IdeZ protease will not cleave non-IgG isotypes, including IgA, IgM, IgD and IgE;

4. IdeZ protease has His tag, which is easy to remove;

5. IdeZ protease can be used in the same reaction with PNGase F (Catalogue No. ATE00005), using the recommended digestion buffer to complete Fc glycans remove.

Guarantee and Disclaimer:

After receiving the product, if finds that the product is mismatched, damaged or missing components, please keep the original package and submit the objection to the company by mail within seven working days. Failure to file an objection within the time limit is considered qualified.

When the buyer keeps the product, it should be kept in accordance with the storage conditions shown in the product label and the manual. If the product quality is caused by improper storage, it will not be guaranteed.

When the buyer tests the product, they should be submitted by the beginning of use when found the quality issue, rather than when the product was used or used up, in order to prepare our products for recycling and confirm the quality of the products. If it is the quality problem, our company is responsible for exchange or return. In the event of a claim, our company will compensate the discretion within the scope of the product price limit and will not accept any part of the value of the product itself. Since the date of receipt, the product has not been reflected for more than three months and should not be returned.

The products provided by our company are for research use only and should not be used for clinical diagnosis or treatment. If a unit or individual changes the use of our products without authorization, we will not bear any responsibility.