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Benzonase Nuclease

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ATE00009
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Product Nameicon
Benzonase Nuclease
Catalog No.icon
ATE00009
Expression hosticon
E.coli
MWicon
29.9kDa
Purityicon
>95% as determined by SDS-PAGE quantitative densitometry by Coomassie Blue Staining
Enzyme activityicon
>800U/μL
Unit Definitionicon
37 ℃, pH 8.0 reaction conditions, 2.625 mL reaction system, change the absorption value of △ A260 to 1.0 in 30 minutes（equivalent to the complete digestion of 37 μg salmon sperm DNA into an oligonucleotide). The amount of enzyme used is defined as an activity. Unit（U).
Tagicon
C-terminal His Tag
Noteicon
For research use only.
Formicon
Liquid
Buffericon
25mM Tris pH 8.0, 100mMNaCI, 50% Glycerol
Storageicon
Store at -20 ° C, 12 months shelf life
1. Method
a. Tissue sample processing method: After grinding 30-100mg of animal and plant tissues, add 100-200ul of lysate, and add 5-10ul of lysate at the same time.
Benzonase Nuclease, mix well, incubate at room temperature or on ice for 30min, collect the lysate, centrifuge and take the supernatant for subsequent operations.
b. Cell sample processing method: Collect suspended cells by centrifugation, resuspend the cells by adding 1ml RIPA lysate, and add 10-20ul of
Benzonase Nuclease, mix well, incubate at room temperature or on ice for 30min, collect the lysate, centrifuge and take the supernatant for subsequent operations.
Tip: Reattach adherent cells to PBS for post-treatment in the same way as suspended cells.
c. Escherichia coli or other bacteria treatment methods: Collect the bacteria by centrifugation and lysate, and add 1-5ul of Benzonase per 1ml
Nuclease, mix well, incubate at room temperature for 30min, collect lysate, centrifuge and take the supernatant for subsequent operations.

    Guarantee and Disclaimer:

    After receiving the product, if finds that the product is mismatched, damaged or missing components, please keep the original package and submit the objection to the company by mail within seven working days. Failure to file an objection within the time limit is considered qualified.

    When the buyer keeps the product, it should be kept in accordance with the storage conditions shown in the product label and the manual. If the product quality is caused by improper storage, it will not be guaranteed.

    When the buyer tests the product, they should be submitted by the beginning of use when found the quality issue, rather than when the product was used or used up, in order to prepare our products for recycling and confirm the quality of the products. If it is the quality problem, our company is responsible for exchange or return. In the event of a claim, our company will compensate the discretion within the scope of the product price limit and will not accept any part of the value of the product itself. Since the date of receipt, the product has not been reflected for more than three months and should not be returned.

    The products provided by our company are for research use only and should not be used for clinical diagnosis or treatment. If a unit or individual changes the use of our products without authorization, we will not bear any responsibility.

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