AtaGenix

DF-1 chicken embryo fibroblast

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Summaryicon
Product Name
DF-1 chicken embryo fibroblast
Organism
Chickens
Age(Gender)
10 days old
Tissue
Embryo
Culture Properties
Adherent growth
Morphology
Fibroblast-like
Background
This cell is a chicken cell line derived from 10-day-old ELL-0 eggs and is spontaneously immortalized. Isolate primary chicken embryo fibroblasts and culture in culture medium; passaging until senescence; centrifuging during aging to keep the cell culture at 30% to 60% full; non-aging clones are identified and passaged no less than 30 times. No clonal proliferation was observed on soft agar, indicating that these cells were immortalized without transformation. The cell can be used as a substrate for virus proliferation, recombinant protein expression and recombinant virus production.
Shipping
Transport at room temperature.
Note
For research use only.
Culture Methodicon
Growth Medium
DMEM high sugar + 10% FBS + 1% P / S
Subculturing
Recommendation 1: 2-1: 3 Passage
Culture Condition
Gas phase: air, 95%; CO2, 5% temperature: 39 ° C(Max.40 ° C, Min.39 ° C)
Cryopreservation Condition
Recommend 92% FBS + 8% DMSO(serum concentration is not lower than 30%, DMSO content is not higher than 12%)

Guarantee and Disclaimer:

After receiving the product, if finds that the product is mismatched, damaged or missing components, please keep the original package and submit the objection to the company by mail within seven working days. Failure to file an objection within the time limit is considered qualified.

When the buyer keeps the product, it should be kept in accordance with the storage conditions shown in the product label and the manual. If the product quality is caused by improper storage, it will not be guaranteed.

When the buyer tests the product, they should be submitted by the beginning of use when found the quality issue, rather than when the product was used or used up, in order to prepare our products for recycling and confirm the quality of the products. If it is the quality problem, our company is responsible for exchange or return. In the event of a claim, our company will compensate the discretion within the scope of the product price limit and will not accept any part of the value of the product itself. Since the date of receipt, the product has not been reflected for more than three months and should not be returned.

The products provided by our company are for research use only and should not be used for clinical diagnosis or treatment. If a unit or individual changes the use of our products without authorization, we will not bear any responsibility.