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rb-EK

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Product Nameicon
Recombinant Bovine Enterokinase(rb-EK)
Catalog No.icon
ATE00002 (OLd ata807)
Expression hosticon
E.coli
MWicon
26.2kDa
Purityicon
>95% as determined by SDS-PAGE quantitative densitometry by Coomassie Blue Staining.
Concentrationicon
> 30 KU / mg,  1mg of recombinant bovine enterokinase can cut at least 1.5g fusion protein.
Unit Definitionicon
At 25 ° C,  reaction buffer 50 mM Tris-HCl (PH 8.0), the amount of enzyme required to digest 50 ug fusion protein containing the digestion site within 16 hours is defined as 1 unit of enzyme activity, which is 1U.
Digestion reaction conditionicon
Digestion at 25 ℃ for 16 hours
Tagicon
C-terminal His Tag
Formicon
Liquid
Buffericon
50 mM Tris-HCl, 50%Glycerol, pH 8.0
Storageicon
Store at -20 ° C, 12 months shelf life
1. During the experiment, please pay attention to :
(1) When the sample contains certain components, it will affect the enzyme activity, such as > 2 M Urea,> 250 mM NaCl,> 20 mM β-ME,> 0.1% SDS,> 50 mM Imidazol
Dialysis to 50 mM Tris-HCl (pH 8.0);
(2) Phosphate can inhibit the activity of enterokinase, and phosphate buffer should be avoided as the enzyme digestion system;
(3) Place on ice bath as soon as possible after dissolution;
(4) If cann’t use all at once, please prepare it into a mother liquor and dispense it into small aliquots (guaranteed the amount of one test) before use.
(5) Avoid the use of strong acids, strong bases, strong oxidants, high concentrations of organic solvents and other reagents that easily cause protein denaturation.

Guarantee and Disclaimer:

After receiving the product, if finds that the product is mismatched, damaged or missing components, please keep the original package and submit the objection to the company by mail within seven working days. Failure to file an objection within the time limit is considered qualified.

When the buyer keeps the product, it should be kept in accordance with the storage conditions shown in the product label and the manual. If the product quality is caused by improper storage, it will not be guaranteed.

When the buyer tests the product, they should be submitted by the beginning of use when found the quality issue, rather than when the product was used or used up, in order to prepare our products for recycling and confirm the quality of the products. If it is the quality problem, our company is responsible for exchange or return. In the event of a claim, our company will compensate the discretion within the scope of the product price limit and will not accept any part of the value of the product itself. Since the date of receipt, the product has not been reflected for more than three months and should not be returned.

The products provided by our company are for research use only and should not be used for clinical diagnosis or treatment. If a unit or individual changes the use of our products without authorization, we will not bear any responsibility.

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