TMB substrate

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Summaryicon
Standard Operating Procedure
Operation procedure
1. Add 100μl TMB to each well.
2. Incubate at room temperature or 37 ° C for 5-30 minutes, and adjust the reaction time according to the reaction system.
3. Add 50μl stop solution (1M hydrochloric acid or 1M sulfuric acid).
4. Test within 30 min., the detection at 450nm.

Notice
1. Sensitive to various oxidants and avoid contamination during the procedure.
2. Aliquot is suggested, using clean plastic containers.
3. High concentrations HRP will cause TMB substrate to appear dark green, or precipitate after adding stop solution, and the amount of antibody should be reduced or the incubation time should be shortened.
Description
The product is ready-to-use and is used for ELISA with the marker horseradish peroxidase; It is a single component, with ultra-stable, high sensitivity, low background, easy to use and so on.The main component of TMB is 3,3 ', 5,5'–tetramethylbenzidine (3,3', 5,5'-tetramethylbenzidine (TMB)), which is a substrate of horseradish peroxidase (HRP) .Under the action of horseradish peroxidase, TMB reacts with an oxidant to produce a blue product. The strength of the color is proportional to the activity of HRP, which can be used for detection based on HRP markers.
Storage
Store at 2-8 ℃ for 2 years.
Shipping
Transport at normal temperature (do not exceed 37 ℃).
Note
For research use only.

Guarantee and Disclaimer:

After receiving the product, if finds that the product is mismatched, damaged or missing components, please keep the original package and submit the objection to the company by mail within seven working days. Failure to file an objection within the time limit is considered qualified.

When the buyer keeps the product, it should be kept in accordance with the storage conditions shown in the product label and the manual. If the product quality is caused by improper storage, it will not be guaranteed.

When the buyer tests the product, they should be submitted by the beginning of use when found the quality issue, rather than when the product was used or used up, in order to prepare our products for recycling and confirm the quality of the products. If it is the quality problem, our company is responsible for exchange or return. In the event of a claim, our company will compensate the discretion within the scope of the product price limit and will not accept any part of the value of the product itself. Since the date of receipt, the product has not been reflected for more than three months and should not be returned.

The products provided by our company are for research use only and should not be used for clinical diagnosis or treatment. If a unit or individual changes the use of our products without authorization, we will not bear any responsibility.