AtaGenix provides one-stop prokaryotic expression service. You only need to provide the gene sequence, we can help you start with gene design, optimize genes, gene synthesis, subcloning and perform protein expression, amplification and target protein purification.

Expression vector: 2-3 kinds of expression vectors, His tag and Sumo or GST tag

  Testing different expression strains and different induction conditions, comprehensive selection of optimal expression conditions

Optimization of expression conditions: induction time, induction temperature and IPTG concentration

-If the protein is soluble, it can be expressed and purified directly

-If the protein is expressed as inclusion body, it can be expressed, purified and refolded.

1. If the customer needs the detailed purification process, including purification conditions and results for each step, an additional fee will be charged. The specific charges will be negotiated according to different project conditions.

2. The expression level and purification yield of each recombinant protein are different and the standard delivery package is 3 mg.

3. The final product, if not specific required, is generally with the solution buffer (Tris-HCl or PBS) and does not contain a denaturing agent such as urea or guanidine hydrochloride. The biological activity of the final product is not guaranteed, but promises that the target protein will be well prepared to protect its activity.