Baculovirus expression vector system is a widely applicable method since it has the advantages of easy screening, post-translational modification and high expression level. With the modified classic Bac-to-Bac expression system, AtaGenix has a rich experience in selecting the means of expression (intracellular or secretory), signal peptide, cell line (Sf9, Sf21, Hi-5) and the corresponding culture method. AtaGenix can offer protein expression service to clients from domestic and overseas pharmaceutical companies, universities, and the with up to a 200 L production batch volume
1. High success rate: AtaGenix has never been unsuccessful using the baculovirus expression vector system.
2. Sophisticated design and screening system: Determine intracellular or secretory expression based on the nature of the protein, and select highly secretory signal peptide for clients. Cell line: Sf9, Sf21, Hi-5, etc. AtaGenix explore the optimum expression conditions to get maximum expression amount by detect multiplicity of infection (MOI) at different time points.
3. Large-scale production: AtaGenix can do production runs of 200 L in protein expression and purification simultaneously.
4. One-stop service: Design and optimize gene, virus production, express and optimize protein, protein purification and post processing, management of data and report.
Service Name | Service Contents | Lead Time | |
Selection of expression system | Expression vector & Cell line |
| |
Construction of the plasmid | Gene optimization & Gene synthesis & Subclone | 2-4 weeks | |
Expression & Purification test | Plasmid transformation and Bacmid identification, P1 virus production and expression test (SDS-PAGE and/or WB), P2 virus production and MOI test (SDS-PAGE and/or WB), 200ml expression and purification test | 3-5 weeks | |
1-10L Expression & Purification | Protein affinity purification (or other purification method), protein buffer replacement and concentration, SDS-PAGE/WB identification of the purified protein | 1-3 weeks | |
Large-scale fermentation & purification | Protein affinity purification (or other purification method), protein buffer replacement and concentration, SDS-PAGE/WB identification of the purified protein | 10-20L | 2-4 weeks |
20-50L | 3-6 weeks | ||
Above 50L | 4-12 weeks |
In this case Protein B using our custom 1 L protein expression and purification package was ordered and AtaGenix found that production of Protein B was 368 mg/L after purification (meeting the requirements of customer on production). Client's feedback showed perfect protein activity. Due to the high protein expression, AtaGenix was then able to provide 60 mg protein to the client and refunded for the 1 L expression and purification.
Consultation and ordering:
Tel: 0086-27-8743 3958
Email:info@atagenix.com