Yeast Expression System
Yeast Expression System
Yeast Expression System is a widely applicable method in industrial microorganism production due to its genetic and physiological background being well known. It combines the advantages of prokaryotic expression and eukaryotic expression for example it is fast to grow, inexpensive to culture, easy to operate, etc. Using yeast, it is possible to perform post-translational modifications, processing and folding, resulting in a recombinant protein which has a more soluble and stable nature.
AtaGenix Advantages
1.The expression hosts include Saccharomyces cerevisiae and Pichia pastoris, and has a high success rate of protein expression.
2.Multiple yeast expression vectors: auxotrophic expression vector for the secretory and intracellular expression, and the high efficient screening expression bacteria strain vector can be transformed to wild type pichia pastoris.
3.Rich experience in yeast expression: Select suitable expression vector based on our client's requirement, explore and optimize the conditions of the whole expression system for customers.
4.High-throughput yeast transformation screening platform, rich experience in yeast protein expression: Many prokaryotic expressed secreted proteins will be insoluble or inactive in inclusion bodies. The yeast expression system can avoid these problems and successfully express proteins.
5.Large-scale production: 1~4000L per batch.
6.One-stop service: Design and optimize the gene, virus production, express and optimize the protein, process the protein post-purification, deal with the data and write the experimental report for the customer.
service content
Service Name | Service Content | Leading Time | |
Selection of expression system | Expression vector & Bacteria strain |
| |
Construction of the plasmid | Gene optimization, gene synthesis & subclone | 2-4 weeks | |
Expression & Purification test | Plasmid linearization, transformation, screening by antibiotic, chose 5-10 positive clone, expression analysis and identification, 100mL amplification and purification test | 3-6 weeks | |
1-10L Expression & Purification | Protein affinity purification (or other purification method), protein buffer replacement and concentration, SDS-PAGE/WB identification of the purified protein | 2-3 weeks | |
Large-scale fermentation & purification | Protein affinity purification (or other purification method), protein buffer replacement and concentration, SDS-PAGE/WB identification of the purified protein | 10-20L | 3-4 weeks |
20-50L | 4-5weeks | ||
Above 50L | 6-12 weeks | ||
Service package
One plasmid construction, test 20 positive clones, the best clone for purification test
Advanced package ata-1032：
One plasmid construction, after testing 20 positive clones, the best 2 clones are selected for optimization of expression conditions, and the best clones and optimal expression conditions are tested for purification.
Screening package ata-1033：
One plasmid construction, the antibiotics are used for screening after transformation. 10 clones are selected for expression strip test. The best two clones are selected for optimization of expression conditions, and the best clones and optimal expression conditions were used for purification test.
Case study
Consultation and ordering：
Tel: 0086-27-8743 3958
Email：info@atagenix.com
- Telephone：Technical：027-87433958 Marketing：027-65279366
- Mailbox：Technical:Info@atagenix.com Marketing:Sales@atagenix.com
- Address：Building C,No.666,Shen Dun Si Lu,Wuhan,430076,CHINA
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