AtaGenix with professional team, perfect protein expression systems, provides a variety of protein purification and identification services, one-stop service to meet the diverse needs of customers
2. Protein identification:
(3) Label excision protease: Thrombin (Leu-Val-Pro-Arg▼Gly-Ser),
Myc, highly specific, uses Myc-tagged peptides to compete with recombinant proteins under natural elution conditions to gently elute Myc-tagged proteins.
MBP, which enhances protein solubility, masks toxic proteins, has a large label and has an effect on protein structure/function.
GST, which enhances protein solubility, only purifies soluble proteins, masks toxic proteins, and has a large label.
6 his, preferred label for protein purification, has small molecular weight and no effect on the protein, can purify soluble/inclusion body proteins.
(2) Purification label:
(1) Purification: chromatography, ion exchange (depending on the nature of protein dots), hydrophobic chromatography (according to hydrophobicity), affinity chromatography (depending on ligand properties)
SDS-PAGE, HPLC, WB, protein concentration detection, absorption spectroscopy