Technical Service

Protein purification and identification

Protein purification and identification

  AtaGenix with professional team, perfect protein expression systems, provides a variety of protein purification and identification services, one-stop service to meet the diverse needs of customers

service content

蛋白纯化流程

2. Protein identification:

rTEV (Glu-Asn-Leu-Tyr-Phe-Gln▼Gly).

Enterokinase (Asp-Asp-Asp-Asp-Lys▼),

(3) Label excision protease: Thrombin (Leu-Val-Pro-Arg▼Gly-Ser),

Myc, highly specific, uses Myc-tagged peptides to compete with recombinant proteins under natural elution conditions to gently elute Myc-tagged proteins.

MBP, which enhances protein solubility, masks toxic proteins, has a large label and has an effect on protein structure/function.

GST, which enhances protein solubility, only purifies soluble proteins, masks toxic proteins, and has a large label.

6 his, preferred label for protein purification, has small molecular weight and no effect on the protein, can purify soluble/inclusion body proteins.

(2) Purification label:

(1) Purification: chromatography, ion exchange (depending on the nature of protein dots), hydrophobic chromatography (according to hydrophobicity), affinity chromatography (depending on ligand properties)


Protein purification:

SDS-PAGE, HPLC, WB, protein concentration detection, absorption spectroscopy

蛋白纯化案例

普健生物的优势

1. 多种蛋白纯化与鉴定方法灵活选择

2. 蛋白质量有保证

3. 一对一项目管理,专业的技术服务团队

咨询与订购

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