TA clones have terminal transferase activity using Taq polymerase, but do not have 3'~5' exonuclease activity, so a non-template dependent base "A" is added to 3' end of the PCR product.
T vector is a highly efficient PCR product cloning vector with a prominent "T" tail at 3' end of T vector, which can be linked to "A" tail of PCR product, improving cloning efficiency.
1. PCR.
2. TA connection.
3. Transformation screening.
4. Sequencing.
1. 2 ug plasmid.
2. Glycerol bacteria.
3. Sequencing map.
4. TA clone experiment report
Cat. No. | Content | Leading time | Price ($) |
ata-G003 | Less than 1500bp | 5,working days | Enquire |
1500bp~3000bp | 5~10 working days | Enquire | |
More than 3000bp | 5~10 working days | Enquire |
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Email:info@atagenix.com
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